Main molecular biology reagents and culture media_Kain Industrial Additives

using the e.coli jm109 genome as a template, using pqe-nlpe-f and pqe-nlpe-r, pqe-spy-f and pqe-spy-r as primers respectively, 2 genes were amplified through high-fidelity pcr fragments, sizes were 711 bp and 486 bp respectively. pcr program: 95°c for 5 min; 98°c for 10 s, 55°c for 15 s, 72°c for 1 min, 30 cycles; 72°c…